Chemosensory mechanisms of host seeking and infectivity in skin-penetrating nematodes.

Approximately 800 million people worldwide are infected with one or more species of skin-penetrating nematodes. These parasites persist in the environment as developmentally arrested third-stage infective larvae (iL3s) that navigate toward host-emitted cues, contact host skin, and penetrate the skin. iL3s then reinitiate development inside the host in response to sensory cues, a process called activation. Here, we investigate how chemosensation drives host seeking and activation in skin-penetrating nematodes. We show that the olfactory preferences of iL3s are categorically different from those of free-living adults, which may restrict host seeking to iL3s. The human-parasitic threadworm Strongyloides stercoralis and hookworm Ancylostoma ceylanicum have highly dissimilar olfactory preferences, suggesting that these two species may use distinct strategies to target humans. CRISPR/Cas9-mediated mutagenesis of the S. stercoralis tax-4 gene abolishes iL3 attraction to a host-emitted odorant and prevents activation. Our results suggest an important role for chemosensation in iL3 host seeking and infectivity and provide insight into the molecular mechanisms that underlie these processes.

More parasitic myositis cases in humans in Australia, and the definition of genetic markers for the causative agents as a basis for molecular diagnosis.

Since 1998, there have been six reported human cases of myositis in Australia, attributable to infection with the nematode Haycocknema perplexum. However, an unequivocal diagnosis of H. perplexum infection and associated disease has been seriously compromised by a lack of molecular markers for this nematode. Here, we report new cases of disseminated myositis in two male patients from the states of Queensland and Tasmania in Australia, respectively; genetically characterize the causative agent from each case; and, also establish a PCR-based sequencing approach as a tool to support the diagnosis of future cases and to underpin epidemiological studies.

Thelaziosis ocular, una zoonosis emergente en España / Ocular thelaziosis, an emergent zoonosis in Spain

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Calving season is a stronger determinant of worm burdens in pasture-based beef production than the level of residual larval contamination at turnout.

The combined influence of (1) calving period (early or late) and (2) overwintering contamination by residual infective larvae (high or low) on subsequent exposure of suckler calves to gastrointestinal nematodes was investigated. We found that the effect of calving date was greater than the level of residual contamination. This was because the adult cows produced large quantities of manure containing small amounts of nematode eggs from turnout, which significantly contaminated the pasture, and thereby, reduced the effect of prior high-low contamination. Early born calves were found to be more heavily exposed to parasites, most likely due to ingesting more herbage than those born later. Late-born calves also had relatively high antibody levels at turnout, which first decreased and then increased again. We suggest that the high antibody levels at turnout reflect passive transfer of maternal antibodies through the milk. There was also a significant difference in animal performance, with the more heavily exposed early born calves having significantly lower daily weight gain than the late-born calves. However, this might not be entirely due to increased parasitism.

Wastewater quality and the risk of intestinal nematode infection in sewage farming families in hyderabad, India.

Use of sewage or wastewater in agriculture is becoming increasingly common as a result of a global water scarcity. Intestinal nematode infections have been identified as the main health risk associated with this practice. To protect consumer and farmer health, the World Health Organization (WHO) has established an intestinal nematode water quality standard. However, because of a lack of well-designed studies, the validity of this guideline is questioned. This report presents the findings of a study on the risk of intestinal nematode infections in farming families occupationally exposed to untreated and partially treated wastewater in Hyderabad, India. The study found an increased risk of hookworm (odds ratio [OR] 3.5, 95% confidence interval [CI] = 2.2-5.5), Ascaris lumbricoides (OR = 5.3, 95% CI = 2.0-14), and Trichuris trichiura (OR = 5.6, 95% CI = 1.8-18) infection when untreated wastewater (150 intestinal nematode ova/liter) was used for crop production. Use of partially treated wastewater (28 intestinal nematode ova/liter) was only associated with an increased risk (OR = 3.2, 95% CI = 1.2-8.6) of A. lumbricoides infection. The findings of the study suggest that the current WHO intestinal nematode guideline of 1 ova/liter is sufficient to protect farmer health.

A sampling of factors relative to the epidemiology of gastrointestinal nematode parasites of cattle in the United States.

Gastrointestinal nematodosis of cattle is a parasitic condition resulting from an immense and seemingly forever-expanding array of factors. Countless determinants influence the incidence and severity of the species-specific infections that occur in cattle, determinants that affect the free-living or environmental stages of the parasites and the parasitic stages. The vast majority of animals have a subclinical or economic level of parasitism undetectable to the eye but quantified more accurately by treatment-induced improved performance (e.g., feed efficiency, nitrogen balance, weight gain, milk production). Unfortunately, the results of treatment (effectiveness and improved animal performance) sometimes can be as varied as the parasitisms that are being treated.

Assessment of the health hazards associated with wastewater reuse: transmission of geohelminthic infections (Marrakech, Morocco).

To address the problem of water scarcity, wastewater has long been used for agricultural purposes in Marrakech (Morocco) and its benefits have long been recognized. However, the benefits of urban wastewater reuse may be limited by its potential health hazards associated essentially to the danger of transmission of pathogenic organisms to human beings. The current study aimed to evaluate the risks associated with raw wastewater reuse for agricultural purposes, and the incidence of geohelminthic infections among the children of the wastewater spreading area of Marrakech as compared to a control group. Faecal samples from 610 individuals were examined for Ascaris spp. ova and Trichuris spp. ova (323 children for the exposed group and 287 for the control group). The study duration was two months and a half, and was carried out in the summer period. A questionnaire-interview with children and parents was used to collect data on possible demographic, hygiene and behavioural-contact factors such as gender, age, family size, source of water supply, parental occupation. The investigations resulted in the detection of Ascaris spp. ova in 43 samples indicating a point-prevalence of 13.3% in the exposed group versus 1.7% for the control group. Trichuris spp. ova was detected in 43 faecal samples indicating a point-prevalence of 13.3% in the exposed group, and 3.8% in the control group. The Chi-square test showed that the differences observed between the exposed and control group are statistically significant (p < 0.01). Males are more infected with ascariasis and trichuriasis than females and children of 2-8 years of age are heavily infected as compared to 9 - 14-year-old children. Based upon the findings of this study, it is concluded that wastewater reuse for agricultural purposes presents an increased risk of infection in the exposed group.

Prevalence and risk factors for protozoan and nematode infections among children in an Ecuadorian highland community.

In order to create a parasite prevention program for Santa Ana, Ecuador, we worked with local physicians to identify the prevalence and risk factors for intestinal parasitic infection among children. Two hundred households were randomly selected and asked to submit three stool samples from a selected child. Information from direct observation and questionnaires was used to determine environmental and behavioral risk factors for parasitic infection. Prevalence of any parasitic infection was 65.6% of the 189 children who submitted fecal samples. Parasitic infections included Entamoeba histolytica/E. dispar (46.6%), Giardia lamblia (18.5%) and Ascaris lumbricoides (20.1%). In a multivariate logistic model, risk factors (P<0.05) for E. histolytica/E. dispar included >1 month since antiparasitic treatment, not adding chlorine to drinking water, children not washing their hands before eating, and having a fewer number of electrical appliances in the home. The risk factor identified for G. lamblia was children not washing their hands before eating. Risk factors for A. lumbricoides included >6 months since antiparasitic treatment and having a fewer number of electrical appliances in the home. This study may have implications for other communities throughout the developing world.

Parasitic diarrhea in normal and malnourished children.

Diarrhea is only one of the many manifestations of intestinal parasites. Environmental influences are inescapable, regardless of an individual's state of health: in a highly endemic region, intestinal parasitic colonization is almost the rule. The clinical expression of the parasitoses, however, is largely determined by host defenses; and when they are weakened, parasitic diarrhea is frequent and severe. Protein-energy malnutrition is by far the most important cause of immune deficiency in developing countries. Diarrhea caused by Strongyloides or Giardia is common and severe in malnourished children, while well-nourished children remain healthy carriers. These parasites require specific treatment in the malnourished; and the well-nourished should have preventive treatment when they are to receive corticosteroids or immunosuppressive agents. Diarrhea caused by Cryptosporidium spp. may be severe in malnourished or immunodeficient children, and recovery is achieved only after renutrition or treatment of the immunodeficiency.

Comparison of nematode infections of the gastrointestinal tract in Angora and dairy goats in a rangeland environment: relations with the feeding behaviour.

A study was conducted in order to examine the possible influence of feeding behaviour on differences in the diversity and the intensity of nematode infections of the gastrointestinal tract between two breeds of goats placed in the same rangeland environment. Within the same flock bred on rangeland conditions, 10 Saanen dairy goats and 14 Angora goats were surveyed for 5 months (from May to September) and parasitological and behavioural parameters were measured. The level of nematode infection was estimated by monthly measurements of faecal egg counts whereas the feeding behaviour was assessed every fortnight. During the whole survey, nematode egg excretion was repeatedly higher in the Angora goats compared to the Saanen ones and significant differences were observed in May and September. This difference in egg excretion was related to major difference in feeding behaviour between the two breeds. In contrast with the Saanen does, Angora goats did not show any browsing and resting activities at any date of observation. Hence, Angora goats can be considered mainly as grazers whereas Saanen goats exhibit feeding activities close to browsers. The relative influence of feeding behaviour and host immune response on the susceptibility to gastrointestinal nematode infection in goats is discussed.

Influence of host weight, sex and reproductive status on helminth parasites of the wild rabbit, Oryctolagus cuniculus, in Navarra, Spain.

A study was carried out in Navarra (northern Spain) on the influence of the weight, sex and reproductive status (lactant, pregnant or lactant + pregnant females and testicular weight for males) of the wild rabbit (Oryctolagus cuniculus) on two cestodes species: Andrya cuniculi and Mosgovoyia ctenoides and four intestinal nematodes: Graphidium strigosum, Trichostrongylus retortaeformis, Nematodiroides zembrae and Dermatoxys hispaniensis. A significantly higher prevalence of A. cuniculi was detected in lactant + pregnant females compared with non-breeding females. Trichostrongylus retortaeformis and N. zembrae showed a significantly higher mean intensity in lactant and lactant + pregnant females than in non-reproductive females. Trichostrongylus retortaeformis presented a higher mean intensity in females than in males, and the mean intensity of the same parasite species was significantly lower in active and inactive males compared with lactant and lactant + pregnant females. There were no significant differences between sexes in the prevalence of helminth parasites. No significant correlation was detected between host weight and the intensity (of infection) of helminths studied. No significant differences in the prevalence and mean intensity of the two cestode species were observed in the three weight categories studied (kittens, juveniles and adults). The prevalence of G. strigosum and mean intensity of T. retortaeformis were significantly higher in older heavier animals than in juveniles.

Comparative susceptibility of Pyrenean and Brown Swiss calves to gastrointestinal nematodes in subclinical naturally acquired infections.

The susceptibility of two common breeds of cattle in the Pyrenees, Brown Swiss and Pyrenean breed, to parasitism by gastrointestinal nematodes was studied. Twenty-two female calves (13 Brown Swiss and 9 Pyrenean), 3 months old with a mean weight of 112.8 +/- 18.33 and 103.3 +/- 17.11 kg respectively at the start of the study, were used. The calves began their first grazing season grazing with 200 cattle following the traditional grazing system for mountainous areas: Stabling during winter (December-April), grazing on harvesting meadows at an elevation of 900 m in the spring (May-June) and autumn (October-November), and grazing areas between 1200-2100 m during the summer (July-September). Faeces, blood samples and calves growth rate were taken at 2-week intervals throughout the grazing season. Faecal egg counts of gastrointestinal nematodes, level of serum pepsinogen, total serum protein and blood parameters were measured. The worm egg counts and Cooperia sp. counts were significantly smaller in the calves of Pyrenean breed than in Brown Swiss throughout the experiment. Numbers of eggs of the other genera of parasites found were smaller in the calves of Pyrenean breed than in calves of Brown Swiss breed, but no statistical differences were found. Blood was compared during two periods: the prepatent and patent periods. During the first period, no differences were found between the breeds for any parameter studied. However, during the patent period, Pyrenean animals had significantly greater numbers of erythrocytes, haemoglobin values and packed cell volume (PCV) values, and smaller numbers of eosinophils than animals of the Brown Swiss breed. No differences were found in the level of serum pepsinogen, total serum protein and live weight gains between the two breeds.

Studies on caprine responsiveness to nematodiasis: segregation of male goats into responders and non-responders.

Eighty-three 2-4-year-old intact male goats exposed to a combination of artificial and natural challenge were segregated into responders and non-responders by ranking of weekly faecal egg counts (FECs). Retrospective analysis of samples over a 15-week-period showed responders had a statistically lower mean FEC than non-responders. Estimates of repeatability between consecutive egg counts were significant in both groups. The 6 top responders and bottom non-responders were subsequently given an artificial trickle challenge with Teladorsagia circumcincta and Trichostrongylus vitrinus. Mean faecal egg output was significantly lower in responders than non-responders. Peripheral eosinophil numbers following challenge were significantly greater in responder than non-responder goats. Abomasal and intestinal worm burdens were considerably lower in responders, with evidence of retardation of worm development compared to non-responders. Both abomasal and jejunal tissue eosinophil numbers were significantly higher in responders, although there was no difference in mucosal mast cell or globule leucocyte numbers. These results suggest that under temperate climatic conditions, it is possible to segregate male goats into responders and non-responders on the basis of simple parasitological criteria.

Studies on helminthosis at the Centre for Tropical Veterinary Medicine (CTVM).

The research work relating to helminths, which has been conducted within the Helminthology Section of the CTVM, often in collaboration with colleagues from the tropics is reviewed and placed into a historical perspective. The research has, in the main, concentrated on the trematodes Fasciola hepatica and Fasciola gigantica and the cestodes Taenia saginata and Taenia solium, but work on other parasites including gastro-intestinal nematodes is also considered. All of these parasites are of obvious veterinary/economic importance particularly in the tropics and subtropics. While the zoonotic importance of T. saginata and T. solium has been recognised for many years, it is only more recently that the zoonotic impact of Fasciola spp. has been generally acknowledged.

Comparative effects of nematode infection on Bos taurus and Bos indicus crossbred calves grazing on Argentina's Western Pampas.

Breed differences in resistance or tolerance to naturally acquired gastrointestinal nematodes were compared between Aberdeen Angus (AA) and Santa Gertrudis (SG) weaned steer calves grazing in a temperate environment. Within breeds, one half of the calves was untreated: AAU (n = 17) and SGU (n = 18) while the other half was treated with moxidectin monthly: AAT (n = 14) and SGT (n = 14). All calves were grazed on the same contaminated pasture from autumn to winter, when each group was separated to uninfected paddocks for the remaining period of the study. Faecal egg counts, plasma pepsinogen levels (PPL), herbage larval counts and liveweight (LW) were recorded monthly. Egg counts and PPL of AAU and SGU increased from autumn to early winter when calves showed heavy parasitism (mainly Trichostrongylus axei) and severely sick calves needed emergency treatment. On the clean paddocks, parasitological parameters progressively decreased. Late winter egg counts were higher (P < 0.05) in SGU than in AAU. PPL of SGU were higher than AAU ones, but no significant differences were detected. Larval differential counts of SGU showed fewer Cooperia (P < 0.08) and higher T. axei (P < 0.002) proportions than those of AAU. Numbers of severely sick and dead SGU calves (61%) were greater (P < 0.01) than those numbers of AAU calves (17.5%). Cumulative LW gains differences within SG breed were higher (P < 0.0001) than those differences within AA breed (P < 0.003). LW gains of AAU during autumn-winter period were greater (P < 0.002) than those of SGU.(ABSTRACT TRUNCATED AT 250 WORDS)